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Yeast two hybrid

Craig Duncan cgdn at uhura.cc.rochester.edu
Wed Jul 19 19:15:30 EST 1995


Dear Ms. Timblin:
     I am a little confused by you question. The nature of the two
hybrid system implies that the activation domain is present and being
expressed in every cell. Positive signals come from interactions
between the activation domain and the DNA binding domain mediated
by the other portions of the hybrid proteins. Thus PCR of the
activation domain gene or mRNA will not tell you anything useful.



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