Try using Saccharomyces carlsbergensis (uvarum) and its
alpha-galactosidase. This enzyme is induced by galactose and repressed by
For your interest, we have a lab manual coming out in September
(Birkhauser, Boston) which uses this bug for a source of protein and DNA.
The manual goes step by step in a process which involves protein
purification, characterization, probe design, cloning, gene
characterization, and sequence application.
Let me know if you need additional details. Good luck.
Biotechnology Training Institute
672 Rt. 202-206 North
Bridgewater, NJ 08807