In article <b-1307952211410001 at g6290mac1.med.utah.edu>, b (mn) writes:
> I have some tricky questions:
>> Why some yeast agregate? I have transformed several yeast strains with my
> plasmid and now they agregate in liquid CM. The same strain without the
> plasmid (2um, TRP1 selection) does not agregate.
>> Is there some way to advoid the agregation? The cells grow very slowly,
> but in plates they look fine.
>> Thank you very much in advance for any advice.
> Rafael Maldonado
>>Rafael.Maldonado at genetics.utah.eduI suggest that you add 0.05 ml (one drop) of Tween 80 per liter of liquid CM. I
think that this will solve the agregation problem. Good luck. R.Randolph