I am having a question concerning plasmid stability in Yeast.
I am currently using a two hybrid system from Stratagene. They recommend
sequencial transformation with bait following bank vectors. After bait
tranformation they recommend growing transformands in YPD-medium
(non selective conditions). Is this really recommendable? How big are the
chances of spontanous plasmid loss lowering my cotransformation rate?
I would be grateful for hints.
Pleas mail: EHRHARDT at mpimg-berlin-dahlem.mpg.de
Thanks a lot!