I am a french PhD student (CNRS, Montpellier, France) seeking for
with my saccharomyces yeast. (e-mail at 73373.1544 at compuserve.com
I have screened a Xenopus cDNA bank (dihybrid technique) to find
to my bait
I have found 9 clones giving the following phenotype His+/Lac+
After segregation of the clones followed by amplification of the
and transformation in E.coli HB101 to eliminate the bait I tried
the specificity of the interaction by backtransforming these
with my bait or with RAS.
But I lost in this backtransformation the phenotype Lac+/His+ in
though my witness reacts well
what can I do to clear up this mystery?
How can I explain the loss of this phenotype?
How can I test the specificity? thank you -- Nathalie CUEILLE