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Help, 5-FOA selection of trxs

E Li U09668 at UICVM.CC.UIC.EDU
Tue Jul 11 09:39:57 EST 1995


Dear fellow yeasters:
I have a strain with a genomic disruption of a gene using a URA3 marker, and
would like to directly transplace the disrupted gene with an intact copy of the
 gene.  I was thinking about directly selecting the transformants on FOA
medium, and was wondering if anyone out there has ever tried this.  I don't
have a selectable marker for the gene I would like to introduce, but I can
select real transformants as opposed to URA3 revertants, by phenotype.
Does this approach seem reasonable, and can anyone give me any possible tricks
 or hints about this strategy? Please e-mail me directly with any advice. Thank
 you very much.


E Li
Univ. of Ill. at Chicago, Dept. of Biological Sciences
U09668 at uicvm.uic.edu



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