Ian A. York (york at mbcrr.dfci.harvard.edu) wrote:
: How stably are
: plasmids maintained in S cervisiae?
Not stable at all compared to E. coli plasmids.
That is, if you stop selective
: pressure, and assuming that the plasmid isn't expressing something toxic,
: what kind of half-life will it show? Will most of the plasmid be lost
: after 6 hours in culture? 24 hours? A week? Or is it unpredicatable,
: or does it depend on other conditions? I'm specifically thinking of 2
: micron plasmids, but any information would be helpful.
A typical 2micron plasmid, the type used in cloning vectors are lost 3-5%
per division. And even under selection as much as 20% of the cells do
not have the plasmid. The specific numbers differ depending on strain of
yeast, type of marker and context of the 2micron sequence used.