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Plasmid stability

Shin Enomoto shin at biosci.cbs.umn.edu
Fri Jul 7 00:46:06 EST 1995


Ian A. York (york at mbcrr.dfci.harvard.edu) wrote:
: How stably are 
: plasmids maintained in S cervisiae? 

Not stable at all compared to E. coli plasmids.

 That is, if you stop selective 
: pressure, and assuming that the plasmid isn't expressing something toxic, 
: what kind of half-life will it show?  Will most of the plasmid be lost 
: after 6 hours in culture?  24 hours?  A week?  Or is it unpredicatable, 
: or does it depend on other conditions?  I'm specifically thinking of 2 
: micron plasmids, but any information would be helpful.

A typical 2micron plasmid, the type used in cloning vectors are lost 3-5% 
per division.  And even under selection as much as 20% of the cells do 
not have the plasmid. The specific numbers differ depending on strain of 
yeast, type of marker and context of the 2micron sequence used. 






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