Hi out there
I need some help from experienced gene-disruptors. I have two genes which I need to interupt, and want to do this by insertion of the URA3 gene. After that I want to be able to use 5-FOA to reselect for a strain which has excised the URA3 gene (but retained my disrupted version of the gene). The genes were both from a library made from the S288C strain, and strains with this background are sensitive to low concentrations of 5-FOA on plates with proline as sole nitrogen source. So ideally I want a diploid yeast strain derived from s288C to do my disruptions. Anyone have an idea which strain to use? Can I get it from the YGSC?
Please post replies or send direct to me.
Thanks
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Colin MacDiarmid
macdiarm at sbsu1.auckland.ac.nz
