Dear yeast netters,
I need to know the chromosomal location of the AXL1 gene described
in the article cited below (since I am interested in some suppressor
gene that resides close to it - get the picture...?). The Saccharomyces
Genome database in Stanford does not have it yet. Can anybody help ?
Thanks in advance !!
Rainer Duden
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==> Fujita A; Oka C; Arikawa Y; Katagai T; Tonouchi A; Kuhara S; Misumi Y.
A yeast gene necessary for bud-site selection encodes a protein similar
to insulin-degrading enzymes.
Nature, 1994 Dec 8, 372(6506):567-70.
Abstract: Cells of the yeast Saccharomyces cerevisiae choose bud sites in a
non-random spatial pattern that depends on mating type: axial for haploid
cells and bipolar for a/alpha diploid cells. We identified a mutant yeast,
axl 1, in which the budding pattern is altered from axial to bipolar.
Expression of the AXL1 gene is repressed in a/alpha diploid cells. With the
ectopic expression of AXL1, a/alpha cells exhibited an axial budding
pattern, thus AXL1 is a key morphological determinant that distinguishes
the budding pattern of haploid cells from that of a/alpha diploid cells.
AXL1 encodes a protein similar in sequence of the human and Drosophila
insulin-degrading enzymes and to the Escherichia coli ptr gene product. The
axial budding pattern might result from degradation of a target protein by
the putative Axl1 protease.
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Dr. Rainer Duden
Molecular and Cellular Biology, U.C. Berkeley
401 Barker Hall, Berkeley, CA 94720
Phone: (510) 642-6171 Fax: (510) 642-7846
Email: Duden at mendel.berkeley.edu
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