>Hi everyone
>I need to do some plasmid shuffle/gene replacement experiments, using the
>5-FOA negative selection for loss of the URA3 marker. This stuff (FOA) is
>horrifically expensive! I have heard a rumour that there is a recipe for
>5-FOA plates which enables one to use a lot less of the compound and get
>the same selection - does anyone out there have a ref for this or any info
>on the technique? post it or mail to me, and I will post a compilation of
>the answers for everyone.
>Thanks,
> colin macdiarmid
>>macdiarm at sbsu1.auckland.ac.nz
UI - 92116644
AU - McCusker JH ; Davis RW
TI - The use of proline as a nitrogen source causes hypersensitivity
to, and allows more economical use of 5FOA in Saccharomyces
cerevisiae.
SO - Yeast 1991 Aug-Sep;7(6):607-8
Michael Lichten
lichten at helix.nih.gov
