RGB12955 at USAV01.GLAXO.COM ("Richard Buckholz") wrote:
>> Does anyone know if the S. cerevisiae PGK promoter works in S. pombe and how
> well? I have a friend who wants to try expressing the same gene in both
> species.
>
cerevisiae promoters in pombe have to be tried. Some do, some dont.
Often they work only very poorly.
Your friend would do better to clone his gene into a pombe vector
(see pombe promoter thread) to make sure it is expressed.
susan
susan_forsburg at qm.salk.edu
