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Constitutive promoter for Sz. pombe expression

TOHDA Hideki htohda at agc.co.jp
Thu Jan 12 18:44:40 EST 1995


In article <3euokp$iu1 at seymour.sfu.ca>, Steven Ritchie <stevenjo at sfu.ca> wrote:

> Hi everyone,
> 
> I am starting up some research with Schizosaccharomyces pombe and need
> some info on expression vectors available for this yeast.
> I am looking to express the pombe G-beta subunit under a constitutive
> promoter in pombe.
> I know already that expression vectors with the ADH or FBP promoters are
> strong, but I will be interested to hear from anyone else about other
> possible systems.
> Any info will be gratefully received, contact : stevenjo at sfu.ca
> 
> Thanks.

Hi Steven,

I am usually using human cytomegalovirus promoter for foreign gene
expression in S.pombe.
The promoter is 10-fold more active than SV40 promoter.
  (Toyama and Okayama, FEBS 268, 217-221 (1990))
I have successfully produced various proteins at a high level (max. 50% of TP).

TOHDA Hideki

-- 
<< TOHDA Hideki / htohda at ddbj.nig.ac.jp / htohda at agc.co.jp >>



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