In article <3euokp$iu1 at seymour.sfu.ca>, Steven Ritchie <stevenjo at sfu.ca> wrote:
> Hi everyone,
>> I am starting up some research with Schizosaccharomyces pombe and need
> some info on expression vectors available for this yeast.
> I am looking to express the pombe G-beta subunit under a constitutive
> promoter in pombe.
> I know already that expression vectors with the ADH or FBP promoters are
> strong, but I will be interested to hear from anyone else about other
> possible systems.
> Any info will be gratefully received, contact : stevenjo at sfu.ca>> Thanks.
Hi Steven,
I am usually using human cytomegalovirus promoter for foreign gene
expression in S.pombe.
The promoter is 10-fold more active than SV40 promoter.
(Toyama and Okayama, FEBS 268, 217-221 (1990))
I have successfully produced various proteins at a high level (max. 50% of TP).
TOHDA Hideki
--
<< TOHDA Hideki / htohda at ddbj.nig.ac.jp / htohda at agc.co.jp >>
