Dear Yeast people,
I am looking for two things,
1) Is there a modern Mitochondrial Transcription Assay, The one I am
currenly using is quite old, and I am concerned about the efficiency.
2) What is the general consensus on the activity of Nuclear-encoded
proteins BEFORE they are transported to the Mito? I would Like to study
a Mito factor but I would rather express is from a plasmid and not have
to do Mito Preps. to get the functional protein.
...oh I should say this is in S. cerevisiae.
Thank you in advance,
Jabed.
