IUBio

PolyA preps and 5' RACE

Hoffmacs Hoffmacs at HERMES.BC.EDU
Wed Jan 11 15:30:17 EST 1995


Hi fellow yeast enthusiasts,
I was wondering whether anyone knows how much of a difference it makes trying
to do 5' RACE from yeast total RNA versus polyA mRNA.  Also, what are your
favorite ways of making the polyA RNA?  What kind of yields can you expect? 
Thanks in advance.
Charlie Hoffman
"I'd rather be fission."



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