Dear Beasties,
I am attempting a 2 hybrid screen for proteins that interact with
a very acidic protein. Unfortunately my LexA fusion seems to be a decent
transcriptional activator on its own. I would like to try 3-AT to reduce
my background of His+ colonies.
I would appreciate your input on how to make up a stock solution
of 3-AT. What is the best solvent to use (H2O?), and what is the maximum
concentration of 3-AT I can get into solution. Thanks in advance for
your help!
Sincerely,
Mike Moser Tel: 206-543-5354
Department of Biochemistry SJ-70 FAX: 206-685-1792
University of Washington moser at u.washington.edu
Seattle, WA 98195 Make peace my beast is yeast
