Recently I mapped a yeast clone to chromosome VI with OFAGE or a similar technique. I have sequence from this clone and it is not in the public databases. I sent the sequence to MIPS and there was no hit in the private databases either. Looking up chromosome VI sequencing plans I find the entire sequence should be in the private database. Why didnt I get a hit on this database? Suggestions to the newsgroup or email me direct please.
Yours in frustration
macdiarm at sbsu1.auckland.ac.nz