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Tetrahydrofolate permeabilization in yeast

NAGARAJAN rajee nagaraj at CS.CONCORDIA.CA
Wed Feb 22 13:18:41 EST 1995


Hello everyone,
        For an enzyme assay I have to use the Tetrahydrofolate as a substrate.
I am planning to suspend the yeast cells in a buffer with 3% brij.  Can this
compound (tetrahydrofolate) get in to the cells?  C



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