Hello out there,
I am interested in preparing TOTAL DNA preps (including chromosomal and
any linear plasmids that may be in the strain) from Saccharomyces cells.
I was wondering if anyone knows whether or not a protocol that is used to
isolate chromosomal DNA (like the one in Current Protocols in Molecular
Biology) will also isolate linear plasmids from the cell. Or are these
two separate protocols?
Responses can be sent directly to me. Thanks in advance!