I have a question about competent cell of yeast. To transform S.
cerevisiae such as w303-1A, I've used frozen competent cells (like E. coli
competent cells) for two years. Efficiency is 10^4 to 10^7 cfu per
microgram pRS313 DNA, depending on the method (LiOAc, spheroplasting, and
electroporation). Recently, one of my friend told me that nobody had
reported a frozen method and that I should report the method somewhere. Is
it true? Is it worth while? I cannot believe that nobody has reported
such a way and that all of yeast people make fresh competent cells
everytime. Please let me know if someone has references.
iizuka at cmews2.med.nagoya-cu.ac.jp (Narushi Iizuka)