On 20 Feb 1995, Narushi Iizuka wrote:
> ... Efficiency is 10^4 to 10^7 cfu per microgram pRS313 DNA,...
I don't know about anyone else, but I would love to find out how
to get 10^7 cfu/mcg of DNA in transformations. The best I've ever gotten
was 2 X 10^5 cfu/mcg and that was a miracle!
We also freeze our cells, although the anecdotally the efficiency
seems to go down somewhat.
Mike Moser Tel: 206-543-5354
Department of Biochemistry SJ-70 FAX: 206-685-1792
University of Washington moser at u.washington.edu
Seattle, WA 98195 Make peace my beast is yeast