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anti-HA immunofluorescence

Anne Spang spang at vms.biochem.mpg.de
Sun Feb 19 10:32:07 EST 1995


Hi!
You could try to optimize the NaCl-concentration in your buffers. For the
12CA5, I usually use up to 250mM salt in PBS and PBS/BSA. Blocking with
preimmune serum also seems to get rid of the background.
Hope this helps!
Anne



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