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anti-HA immunofluorescence

Anne Spang spang at vms.biochem.mpg.de
Sun Feb 19 11:44:20 EST 1995

I have forgotten to mention that completing YP-Media with sterilfilterized
glucose yields in quite an important background reduction, especially when
the 12CA5 is used. If one autoclave YPD, reactions between the glucose and
the yeast extract occur, resulting in already slightly autofluorescent
stuff. This stuff seems also be regognized by 12CA5.
Good luck!

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