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fragments of LEU2 that complement

David Stillman stillman at bioscience.utah.edu
Thu Feb 16 14:02:56 EST 1995


In article <3hvss1$nrp at news.cs.brandeis.edu> Charles Brenner,
brenner at rose.brandeis.edu writes:
>Does anyone know if the 2 kb Hpa I-Sal I fragment of LEU2 complements
>as a single copy integrant?  I know that fragment(s) shorter than the
>2.2 kb Xho I-Sal I fragment are used in leu2d vectors to select for
>high copy number.  What fragments are those and will Hpa-Sal work in a
>knockout?

Yes, the 2 kb Hpa I-Sal I fragment will complement. A smaller fragment
(HpaI-AccI; 1.6 kb) present in the YDp-L vector (Yeast 7:475) complements.

David Stillman
Department of Cellular, Viral and Molecular Biology
University of Utah Medical Center
Salt Lake City, UT 84132
stillman at bioscience.utah.edu
phone: 801-581-5429
fax: 801-581-3607



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