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G-protein effectors

Hoffmacs Hoffmacs at HERMES.BC.EDU
Wed Feb 15 06:15:02 EST 1995

Both S. cerevisiae and S. pombe have two G alpha genes.  The S. cerevisiae
GPA1 gene acts as a negative regulator of the pheromone response pathway
(Dietzel and Kurjan, 1987; Miyajima et al., 1987).  The role of the S.
cerevisiae GPA2 gene is less well characterized (Nakafuku et al., 1988). 
While over-expression of GPA2 suppresses a temperature-sensitive mutation in
RAS2, suggesting a possible role in adenylate cyclase activation, it does not
suppress the lethality of a ras1 ras2 double disruption.  Furthermore,
disruption of the GPA2 gene has no effect upon regulation of adenylate
cyclase.  The S. pombe gpa1 gene is a positive regulator of the pheromone
response pathway (Obara et al., 1991), while the S. pombe gpa2 gene is
required for the production of a glucose-induced cAMP response (Isshiki et
al., 1992).  We have shown that this cAMP response is created by the
activation of adenylate cyclase (Byrne and Hoffman, 1993), and that gpa2 is
identical to git8 (Nocero et al., 1994).  Hope this gives you a starting
Charlie Hoffman
"I'd rather be fission."

I am working with a fungal relative of S. cerevisiae.  I would like to 
determine what the effects of activation and/or inactivation of a
(probable) G-protein mediated transduction pathway might be.  Which effectors
usable with intact cells are known to affect the S. cerevisiae or other
fungal G-alpha proteins.  It has not been trivial to do a specific enough
literature search for this info.  

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