I made a typo in my follow-up. It should read 3% glucose (not 35).
Megan Brown (mbrown at fred) wrote:
: I don't know if it's best, but I use synthetic media, e.g. complete
: synthetic minus hisitidine etc. I autoclave the media first, then let it
: cool some before adding the appropriate amound of 3AT. I make up the 3AT
: in H2O as a 1 M stock and store it at -20. I have found that adding 35
: glucose to the media instead of my usual 2% increases plating efficiency
: on this media, but this is somewhat anecdotal. It is crucial to leave the
: histidine out of the media; thus, I would not expect rich media such as
: YEPD to work.
: Megan Brown
:mbrown at fred.fhcrc.org