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3-AT, YMM? YPD?

Megan Brown mbrown at fred
Wed Feb 15 13:19:00 EST 1995


I made a typo in my follow-up. It should read 3% glucose (not 35).

Megan Brown


Megan Brown (mbrown at fred) wrote:
: I don't know if it's best, but I use synthetic media, e.g. complete 
: synthetic minus hisitidine etc. I autoclave the media first, then let it 
: cool some before adding the appropriate amound of 3AT. I make up the 3AT 
: in H2O as a 1 M stock and store it at -20. I have found that adding 35 
: glucose to the media instead of my usual 2% increases plating efficiency 
: on this media, but this is somewhat anecdotal. It is crucial to leave the 
: histidine out of the media; thus, I would not expect rich media such as 
: YEPD to work.

: Megan Brown
: mbrown at fred.fhcrc.org





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