On 13 Feb 1995 ROSENKRANTZ at gems.vcu.edu wrote:
> Does anyone have an alternative to liquid nitrogen lysis?
> Are xgal plates too unreliable?? THanks
I too wondered about this questions. I tried using X-Gal plates for
2-hybrid strains where colony lifts were recommended, and I got no blue
color at all. I do get good blue color from other strains. It seems that
some 2-Hybrid strains are less "leaky" than others when it comes to
secreting beta-Gal into the media. Thus, for "leaky" strains you can use
X-Gal plates. For "non-leaky" strains you need to do colony lifts. The
lifts do have the advantage of being faster than plates.
Mike Moser Tel: 206-543-5354
Department of Biochemistry SJ-70 FAX: 206-685-1792
University of Washington moser at u.washington.edu
Seattle, WA 98195 Make peace my beast is yeast