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Shun'ichi Kuroda skuroda at inherit.biosig.kobe-u.ac.jp
Fri Feb 10 01:13:34 EST 1995

Dear Paul at CLONTECH:

Thank you very much for your immediate and kind responce on my post.
For the news group readers who have not read the CLONTECH's message,
I have attached below the message you posted to the Biotech.methods
list in last October.

Many Thanks,

In addition to notifying CLONTECH customers directly, we are 
posting this notice on Methods and Reagents because we feel 
it will be the quickest way to convey important information 
concerning our MATCHMAKER Two-Hybrid Supplement (Cat. #K1605-D).  

We recently identified a problem with the histidine reporter 
construct in the cycloheximide resistant HF7c.cyh yeast 
strain contained in the MATCHMAKER Supplement.  Thus, this 
strain cannot be used for Two-Hybrid library screening.  We 
are working to resolve this situation as soon as possible.  
Individuals who have already purchased this system will 
receive notification by mail describing the problem and 
outlining the steps we have taken to correct it.

We would like to reassure all our customers that this situation 
only concerns the cycloheximide resistant HF7c.cyh yeast strain 
in the MATCHMAKER Supplement which was introduced in July of 
this year.  There is no problem with the standard HF7c yeast 
strain described by Feilotter, et al (1994, Nucleic Acids 
Res. 22:1502-1503) and present as a component of the original
MATCHMAKER System (Cat. #K1605-1) that we introduced almost 1 
year ago.  In fact, although the HF7c strain in the MATCHMAKER 
System is not cyclohexamide resistant, it can be used for library 
screening purposes with the pAS2 plasmid contained in the 
MATCHMAKER Supplement.

We apologize for any inconvenience and we are doing everything 
possible to resolve this situation as quickly as we can.  We 
appreciate your understanding and patience.  If you purchased 
the MATCHMAKER Supplement (K1605-D), please contact CLONTECH 
Technical Service at your convenience.  Customers outside the 
U. S. should contact their local distributor.

Thank you,

  >  Paul Diehl, Ph.D.              Technical Service     <
  >  CLONTECH Laboratories          ph: 800-662-2566 x141 < 
  >  4030 Fabian Way                fx: 415-424-1064      <
  >  Palo Alto, CA 94303-9605       tech at clontech.com     <

E-mail: skuroda at inherit.biosig.kobe-u.ac.jp
Tel +81-78-803-1255   Fax +81-78-803-0994

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