We are trying to localize a HA-tagged protein in cerevisiae by using BMB's
anti-mouse monoclonal. We would appreciate that someone who has successfully
done the same or similar (using anti-HA) thing before to provide protocols or
suggestions. I heard that this antibody appears to recognize nonspecific
proteins (containing HA-like epitopes) in the cerevisiae. If so, how can this
be solved? We are thinking about doing a preadsorption to yeast powder. Is
this a good way? Finally, does the strain genetic background matter?
Thanks in advance.
Department of Molecular Genetics
The Ohio State University
484 West 12th Ave.
Columbus, OH 43210
chang.108 at osu.edu