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phloxine B in budding yeast

John Cannon cannon at MEDSCI.MBP.MISSOURI.EDU
Wed Feb 8 19:51:54 EST 1995

Phloxine B can be used for Saccharomyces cerevisiae at 20 mg / liter
added to any medium after autoclaving. It is great for scoring
temperature-sensitive strains or for detecting small differences in viability.
The cells stained on a plate can even be scraped off and examined
microscopically. Colony level staining results from less than 1% of the
cells dying. I started using it a long time ago (see Genetics 113:247-264)

John Cannon
Department of Molecular Microbiology and Immunology
University of Missouri
Columbia, Missouri 65212

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