Kevin Piers writes,
>>Does anyone out there know of a reliable method for preparing yeast
>colonies on NC filters for the purpose of probing them with a specific
>piece of DNA? Any information is greatly appreciated and can be sent
>directly to me.
>I have found that the protocol given in the Cold Spring Harbor Methods in
Yeast Genetics manual (Rose, Winston, Hieter are the authors of my version)
works well. I generally replica plate onto filters on YPD plates, let grow
4-6 hr at 30C, and then lyse as they describe.
lichten at helix.nih.gov