>In article <sjszarka-2001951516100001 at canola.bio.ucalgary.ca> Steven
>Szarka, sjszarka at acs.ucalgary.ca writes:
>>For galactose induction of a GAL10-CYC1 hybrid promoter,
>>how pure does the galactose need to be? Is 1-2% glucose
>>in the galactose stock an acceptable level?
>The GAL10 promoter is repressed by glucose, but the amounts
>you mention are not significant. It should work fine.
I agree - in my experience, nothing is as good at removing trace amounts of
glucose as a yeast culture ! I presume they use up the glucose and then get
derepressed and induce.