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Galactose Purity for GAL10-CYC1 Induction?

Simon Williams s.p.williams at bioc.cam.ac.uk
Sun Feb 5 09:48:39 EST 1995


>In article <sjszarka-2001951516100001 at canola.bio.ucalgary.ca> Steven
>Szarka, sjszarka at acs.ucalgary.ca writes:
>>For galactose induction of a GAL10-CYC1 hybrid promoter,
>>how pure does the galactose need to be? Is 1-2% glucose 
>>in the galactose stock an acceptable level?

>The GAL10 promoter is repressed by glucose, but the amounts
>you mention are not significant.  It should work fine.

>Anthony

I agree - in my experience, nothing is  as good at removing trace amounts of 
glucose as a yeast culture !  I presume they use up the glucose and then get 
derepressed and induce.

Simon Williams
Biochemistry Dept
Cambridge University



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