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TWO-HYBRID; dectection of fusion proteins

wvcrieki at lmb1.rug.ac.be wvcrieki at lmb1.rug.ac.be
Thu Feb 2 19:46:12 EST 1995


Hello ...,

Is there anyone with experience in detecting fusion proteins on SDS-PAGE. I am 
currently using ANTI-GAL4db(1-147) polyclonal antibody to identify fusion 
proteins on a western blot. Any information about preparation of the lysate, 
amount of proteins needed (density of the culture ?) would be appreciated. 
Tips on how to reduce non-specific bands are welome (concentration of 
anti-body, supplier of anti-body, blockage, etc. ?)

Thanks for your time

Wim Van Criekinge
E-mail: WVCRIEKI at LMB1.RUG.AC.BE 



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