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spheroplast regeneration

charlie brenner brenner
Fri Dec 22 13:34:21 EST 1995

Jos Urbanus wrote:
>To produce spheroplasts from normal cells is very easy, but until now I
>have not been able to regenerate these cells. In an 0.7 M sorbitol
>YNB-medium spheroplast do not generate a cell wall, produce enormous
>vacuoles and finally blow themselves up, I guess.

You can't regenerate spheroplasts in liquid culture.  If your medium has been
liquid, you'll have to switch to a top agar with sorbitol or gently plate on
sorbitol-containing plates.

Charlie Brenner
Rosenstiel Center, rm 650	phone (617) 736-4908
Brandeis University		fax   (617) 736-2405
Waltham, MA 02254-9110		brenner at auriga.rose.brandeis.edu

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