Jos Urbanus wrote:
>To produce spheroplasts from normal cells is very easy, but until now I
>have not been able to regenerate these cells. In an 0.7 M sorbitol
>YNB-medium spheroplast do not generate a cell wall, produce enormous
>vacuoles and finally blow themselves up, I guess.
You can't regenerate spheroplasts in liquid culture. If your medium has been
liquid, you'll have to switch to a top agar with sorbitol or gently plate on
sorbitol-containing plates.
Charlie Brenner
--
Rosenstiel Center, rm 650 phone (617) 736-4908
Brandeis University fax (617) 736-2405
Waltham, MA 02254-9110 brenner at auriga.rose.brandeis.eduhttp://www.rose.brandeis.edu/users/brenner
