In article <Pine.A188.8.131.521218175611.65985B-100000 at spider.usp.br>,
fgdnobre at usp.br (Francisco G da Nobrega ib - bio 7588) wrote:
> Dear yeast people:
>> I would like to have advice on the best way to keep for long time =20
> sporulated S. cerevisiae diploids suitable for sampling, glusulase=20
> treatment and spore analysis.
> Does it keep well in liquid sporulation medium?
> Is it possible to freeze away at -80oC (plus glycerol) without rupture of=
> the ascus wall?
I have kept ascii in liquid sporulation medium for a couple of weeks at
4oC with no apparent ill effects.
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