Hi, everybody
I am from the biochemical department of the VU, Amsterdam, and trying to
develop different kinds of toxicity assays with yeast. In order to
increase the availability of a drug to a cell, spheroplasts can play an
important role in combination with certain kind of drugs. In such an
assay it would be essential that most cells stay alive and keep on
proliferating.
To produce spheroplasts from normal cells is very easy, but until now I
have not been able to regenerate these cells. In an 0.7 M sorbitol
YNB-medium spheroplast do not generate a cell wall, produce enormous
vacuoles and finally blow themselves up, I guess.
So, if anybody has experience with spheroplast regeneration, please give
me advice or a protocol.
