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spheroplast regeneration

Michael Lichten lichten at helix.nih.gov
Mon Dec 18 12:48:09 EST 1995

In article <4b3jh7$fnd at trst.cca.vu.nl>, jos urbanus <urbanus at chem.vu.nl> wrote:

> Hi, everybody
> I am from the biochemical department of the VU, Amsterdam, and trying to 
> develop different kinds of toxicity assays with yeast. In order to 
> increase the availability of a drug to a cell, spheroplasts can play an 
> important role in combination with certain kind of drugs. In such an 
> assay it would be essential that most cells stay alive and keep on 
> proliferating.
> To produce spheroplasts from normal cells is very easy, but until now I 
> have not been able to regenerate these cells. In an 0.7 M sorbitol 
> YNB-medium spheroplast do not generate a cell wall, produce enormous 
> vacuoles and finally blow themselves up, I guess.
> So, if anybody has experience with spheroplast regeneration, please give  
> me advice or a protocol.

Try 1 M sorbitol.

Michael Lichten
lichten at helix.nih.gov

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