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Storage of Sporulated culture

Francisco G da Nobrega ib - bio 7588 fgdnobre at usp.br
Mon Dec 18 15:04:06 EST 1995

Dear yeast people:

I would like to have advice on the best way to keep for  long time =20
sporulated S. cerevisiae diploids suitable for sampling, glusulase=20
treatment and spore analysis.
Does it keep well in liquid sporulation medium?
Is it possible to freeze away at -80oC (plus glycerol) without rupture of=
the ascus wall?

Thanks for the advice!


F.G. Nobrega
Universidade de S=E3o Paulo
Biologia - IBUSP
C.P.  11461
05422-970   S=E3o Paulo  -  SP  - Brasil
Voice: (011) 818-7588   Fax: (011) 818-7553    E-mail:  fgdnobre at usp.br

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