Dear yeast people:
I would like to have advice on the best way to keep for long time =20
sporulated S. cerevisiae diploids suitable for sampling, glusulase=20
treatment and spore analysis.
Does it keep well in liquid sporulation medium?
Is it possible to freeze away at -80oC (plus glycerol) without rupture of=
the ascus wall?
Thanks for the advice!
Universidade de S=E3o Paulo
Biologia - IBUSP
05422-970 S=E3o Paulo - SP - Brasil
Voice: (011) 818-7588 Fax: (011) 818-7553 E-mail: fgdnobre at usp.br