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Reporter genes and competative inhibitors

Eddie Diehl diehl001 at mc.duke.edu
Sat Dec 16 12:21:11 EST 1995


In article <rennie-1412951711390001 at gietz.hgen.umanitoba.ca>,
rennie at bldghsc.lan1.umanitoba.ca (S.L. Rennie) wrote:

> Hello fellow yeast-ers
>    I'm looking for a reporter gene that I can use that has some sort of
> chemical and/competitive inhibitor that I can add to the media (much like
> the His3 3-AT set-up).  I don't know of any other "common" reporter genes
> that have this same phenomenon  and I'm not sure where to get biochemical
> pathway info to start looking - - yes I actually dragged myself down to
> the library before I posted this, but yeast aren't a big thing at this
> university I guess 'cause I came up pretty dry.
> 
> Thanks for any response
> sincerely
> Sharon Rennie

Dear Sharon:

I think 6-aza-uracil can be used with a Ura marker. I'll try to find you
some references.

Eddie Diehl



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