IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

yeast two hybrid system

Jochen Strayle jochen.strayle at po.uni-stuttgart.de
Thu Dec 14 15:47:51 EST 1995


I work with the tester strain Y190 and the pAS2 plasmid from Elledge. I
found library plasmids which got specific blue with my fishing plasmid.
But it was not possible to detect any blue colour after retransforming.
Another problem was:Sometimes the library plasmids I found from the blue
colonies had a wrong  backbone. Is there anybody with experience in this
strain and pas2?


More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net