In article <199512140152.RAA23289 at ucdavis.ucdavis.edu>,
kcburtis at UCDAVIS.EDU (Ken Burtis) wrote:
> Has anyone out there found a good method of breaking reasonable
> quantities of Pichia (e.g. cells from 1 liter of culture) ?
> Spheroplasting with Zymolase gets expensive with large quantities, and
> we have been unable to get better than about 5 % breakage using two
> passes at 20,000 psi in a French press.
We use a "bead beater" to break them up. Its made, I think, by BioSpec in
Oklahoma.
You might try calling Invitrogen to see if they can give you a number
where you can find one.
We tried the french press too and it didn't even phase the little buggers.
Kurt
