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Growth of Yeast on Non-Fermentable CHO's

Greg Cosentino Cosentino at medcor.mcgill.ca
Mon Dec 11 18:08:05 EST 1995

Hi Netters,

I am checking the ability of a yeast strain to grow on 
non-fermentable carbohydrates (glycerol, ethanol, and acetate) 
compared to glucose. As a lark, I also streaked my yeast onto 
control plates composed of yeast extract and peptone but with no 
carbohydrate at all. It seems that the strains capable of aerobic 
growth also grew in the absence of any carbohydrate whatsover, but 
the petite mutants didn't grow. The seed cultures were made in 
liquid YPD media and then washed extensively with water prior to 
plating so I wouldn't carry over any of the glucose containing media 
when I plated them. Any ideas to explain growth of the yeast in the 
absence of carbohydrate?

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