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Breaking Pichia cells

Ken Burtis kcburtis at UCDAVIS.EDU
Wed Dec 13 20:54:38 EST 1995

Has anyone out there found a good method of breaking reasonable 
quantities of Pichia (e.g. cells from 1 liter of culture) ?  
Spheroplasting with Zymolase gets expensive with large quantities, and 
we have been unable to get better than about 5 % breakage using two 
passes at 20,000 psi in a French press.

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