dear yeast people,
i need a screen for yeast mortality on agar plates, which is non-toxic
and will distinguish cells in growth arrest from truly dead ones. I have
been trying to use a methylene blue method (Iida et al., 1994), but all my
cells turn blue whether i've threatened their lives or not.
So, does anyone know what exactly methylene blue is staining in dead
yeast, and whether it is toxic over several days?
And, any other bright ideas for easily detecting survival on agar plates
(in the absence of growth)?
thanks very much!