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two hybrid

Guy Oshiro Guy.Oshiro at UCHSC.edu
Wed Dec 6 14:36:32 EST 1995


Someone has done this already using an antiboitic resistance gene as a
marker.  I don't have the paper handy so I can't give you a reference. 
They used their system to just look at possible interactors.  They did
not and I would not recommend trying to use a mammalian system to
screen a library.  You usually need to screen at least about 2 million
transformants in a mammalian library and I don't anyone who would want
to do that many transfections.

Guy Oshiro
EMAIL: Guy.Oshiro at UCHSC.edu

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