In article <30B37DE9.314F at medcor.mcgill.ca>, Greg Cosentino <Cosentino at medcor.mcgill.ca> says:
>>I have what will likely be a rather naive question. A yeast strain I
>am using has the designation "GAL+" in the genotype description. I
>can't find this described in either the Guthrie and Fink or
>Strathern, Jones and Broach yeast handbooks. My guess is that it
>means the yeast galactose regulon is intact (ie. wild-type GAL4 and
>GAL80 genes) and that this strain can utilize galactose. Is this
>right or hopelessly wrong? It is important as I am planning to
>transform this strain with galactose-inducible expression vector.
>Thanking you for your help in advance.
>Greg
Dear Greg:
The "GAL+" genotype means only that your strain is able to growth
on galactose as the only carbon source. It can be a wild-type strain, with
intact GAL4 and GAL80 gene plus all the other enzymes necessary for galactose
catabolism (GAL2, GAL3, GAL1, GAL5, GAL7, GAL10), or it can have a gal80
mutation, which make it express constitutively all the enzyme for galactose
utilization.
I believe that you need utilize a better characterized strain.
Joab.
