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Plasmid integration at ura3-1 locus

Eckhard Boles boles at bio1.bio.th-darmstadt.de
Fri Dec 1 02:22:44 EST 1995

To integrate plasmids into the ura3 locus we always linearize them with 
NcoI. However, we use strains with the ura3-52 mutation (a Ty insertion). 
I don't know the kind of mutation in your ura3-1 allel. You might have 
problems if it is a deletion mutation.

Best regards

Dr. Eckhard Boles
TH Darmstadt
Email: boles at bio1.bio.th-darmstadt.de

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