>jjschm at ccmail.monsanto.com (Jon Schmuke) wrote:
>>>>Does anyone know the best way to titer expression from the Gal1 promoter?
>I don't know what the best way is, but we have had excellent luck growing cells
>galactose with varying amounts of glucose (up to 1%). With 2%/1% gal/glu in
>medium, expression from the GAL1 promoter is reduced 100-fold, as assayed by
>of the expressed protein. This is in a strain that started out as S288C
>but was backcrossed several times to a good Gal+ strain.
>stearns at leland.stanford.edu_______________________________
Tim (and all):
When you add glucose to galactose, don't the cells use up the glucose first, and
hence fully induce themselves when they're finished? Do you have to catch them
real early, before this has happened?