I posted a request for a S.cerevisiae cDNA library earlier today.
One requirement for this library that I failed to include is that
I want the cDNA FUSED to a Gal4 Activation Domain. This vector
would also include a LEU2 Marker and 2um ORI. Also I would like
this to be constitutively expressed...ADHpro/term? I was thinking
maybe something like pGAD424?
I apologize for this confusion. I was getting carried away with a potential
good result and didn't think this through properly.
Dept. of Cell and Molecular Biology
5 Cummington St.
Boston, MA 02215
email: harkness at bio.bu.edu