Dear Yeast.net members:
I have a question concerning the His-tag system for purifying
proteins from yeast. I am thinking of trying to purify a multi-subunit
protein from yeast by tagging one of its subunits with the 6 x His tag.
Has anyone tried this approach? I am concerned about whether or not the
other subunits will stay associated through the purification steps. I
realize that different proteins will likely behave differently, but any
info would be appreciated.
Also, are there anti- 6 x His antibodies out there, and if so, how
good are they?
Thanks for your help,
Michael A. McAlear
Dept. of Molecular Biology and Biochemistry
Hall-Atwater-Shanklin Labs, Rm 228
e-mail: mmcalear at wesleyan.edu