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Titering Gal1 promoter

tim stearns stearns at leland.stanford.edu
Thu Aug 31 17:49:19 EST 1995

>><stuff about regulating GAL promoter with glucose/galactose mixture deleted>

>Tim (and all):
>When you add glucose to galactose, don't the cells use up the glucose first, and
>hence fully induce themselves when they're finished?  Do you have to catch them
>real early, before this has happened?
>Josh Trueheart

That is what I have always heard, and we have never looked in very dense 
cultures (OD > 1).   

Tim Stearns
stearns at leland.stanford.edu 

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